MSC Characterization Antibody Panel
Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells
概要
The MSC Characterization Antibody Panel is designed for the phenotypic analysis of human mesenchymal stromal cells (MSCs). This kit contains three fluorochrome-conjugated antibodies for the identification of human MSCs, and a fluorochrome-conjugated antibody for the identification of non-MSCs. When measured by flow cytometry, ≥ 95% of the MSC population must express CD73, CD90, and CD105, and these cells must lack expression (≤ 2% positive) of CD45. These reagents provide single-step labeling for the verification of human MSCs.The MSC Characterization Antibody Panel can be used to identify human MSCs derived from various tissues such as bone marrow MSCs, pluripotent stem cell-derived mesenchymal progenitor cells, adipose-derived MSCs, dental pulp-derived MSCs, and umbilical cord-derived MSCs.
Subtype
Primary Antibodies
Cell Type
Mesenchymal Stromal Cells
Application
Flow Cytometry
Area of Interest
Endothelial Cell Biology, Stem Cell Biology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | MSC Characterization Antibody Panel | 100-0354 | All | English |
数据及文献
Data
Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel
(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.