EasySep™ Human CD15 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Human CD15 Positive Selection Kit is designed to isolate CD15+ cells from ammonium chloride lysed blood or other nucleated cell suspensions by positive selection. Desired cells are targeted with antibody complexes recognizing CD15 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD15 antigen is expressed on monocytes and granulocytes.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ Human CD15 Positive Selection Kit (Catalog #18651)
    • EasySep™ Human CD15 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human CD15 Positive Selection Kit (Catalog #18651RF)
    • EasySep™ Human CD15 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Myeloid Cells
Species
Human
Sample Source
PMNC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Human CD15 Positive Selection Isolation Profile

Starting with lysed whole blood, the CD15+ cell content of the isolated fraction is typically 98.8 ± 0.8% (gated on CD45+ cells; mean ± SD). In the above example, the purities of the start and final isolated fractions are 50.7% and 98.0%, respectively.

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