EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection
概要
The EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9, CD81, and CD63, and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.​
Advantages
⦁ Fast, easy to use, and column-free.
⦁ Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.
⦁ Obtain highly purified EVs without contaminating biofluid components.
Components
  • EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit (Catalog #17891)
    • EasySep™ Human Pan-Extracellular Vesicle Positive Selection Cocktail 1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
    • NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Other
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Extracellular Vesicle Research
数据及文献

Data

Positive isolation of EVs with CD9, CD63, and CD81 tetraspanin markers from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with CD9, CD63, and CD81 tetraspanin markers from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

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