EasySep™ Human Pan-ILC Enrichment Kit
Immunomagnetic negative selection cell enrichment kit
概要
The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
Advantages
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILCs
• Untouched, viable cells
• Facilitates rapid flow sorting of ILCs
Components
- EasySep™ Human Pan-ILC Enrichment Kit (Catalog #17975)
- EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50103, 1 mL
- RoboSep™ Human Pan-ILC Enrichment Kit (Catalog #17975RF)
- EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50103, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Pan-ILC Enrichment Kit | 17975 | All | English |
| Product Information Sheet | RoboSep™ Human Pan-ILC Enrichment Kit | 17975RF | All | English |
| Safety Data Sheet | EasySep™ Human Pan-ILC Enrichment Kit | 17975 | All | English |
| Safety Data Sheet | RoboSep™ Human Pan-ILC Enrichment Kit | 17975RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile
Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.